I am attempting to recreate an extraction of steroidal glycosides from Lily bulbs from this paper Article Isolation and Structural Determination of Steroidal Glycosid...
To do so I need to run GPC on a very large column packed with Sephadex LH-20. All the tubing is set up and we are about to pack the column, however I'm wondering how to set up the column outlet so that I can control fraction collection. Right now it only has a HDPE bed support. Should I get a PTFE bed support, or do I need to connect tubing to the outlet? The solvent is ethanol and water. Thank you in advance.