I am struggling to get fine cholesterol crystal for cell. I have got cholesterol crystal through the methods from many papers, but I cannot get very small particles from relatively bigger particles. I tried to sonify it but it dosent seem to work - as well the zetasizer shows a huge dispersion in crystals size - I aim dor size 200-300 nm to apply to cell.

I am looking forward to get a advise from you!

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