Michał Żorniak

3 Questions 4 Answers 0 Followers

Questions related from Michał Żorniak

Is it possible to activate macrophages NLRP3 inflammasome without priming?

Hi, is it possible to activate inflammasome in mice BMDM cells wich are not primed with LPS? We are trying to search for increase of Il1ß after cholesterol crystals incubation but we do not see...

02 February 2020 662 2 View

Could anyone share protocol for filipin tissue staining?

I would like to stain slides prepared from biopsies of GI tract for cholesterol (and cholesterol crystals), would you recommend any filipin staining protocol ?

01 January 2020 2,487 2 View

Cholesterol crystals preparation?

I am struggling to get fine cholesterol crystal for cell. I have got cholesterol crystal through the methods from many papers, but I cannot get very small particles from relatively bigger...

10 October 2019 3,302 2 View