ERROR 1 [file lig.itp, line 363]:
No default Proper Dih. types
ERROR 2 [file lig.itp, line 370]:
No default Proper Dih. types
ERROR 3 [file lig.itp, line 390]:
No default Proper Dih. types
ERROR 4 [file lig.itp, line 414]:
No default Proper Dih. types
ERROR 5 [file lig.itp, line 494]:
No default Proper Dih. types
Excluding 3 bonded neighbours molecule type 'Protein_chain_P'
Excluding 3 bonded neighbours molecule type 'lig'
Excluding 2 bonded neighbours molecule type 'SOL'
NOTE 1 [file topol.top, line 35404]:
System has non-zero total charge: -10.000000
Total charge should normally be an integer. See
http://www.gromacs.org/Documentation/Floating_Point_Arithmetic
for discussion on how close it should be to an integer.
WARNING 1 [file topol.top, line 35404]:
You are using Ewald electrostatics in a system with net charge. This can
lead to severe artifacts, such as ions moving into regions with low
dielectric, due to the uniform background charge. We suggest to
neutralize your system with counter ions, possibly in combination with a
physiological salt concentration.
I got this error while generating ions.tpr in gromacs . how to fix this error
Thanks in advance