Can I use asymmetric PCR amplification on a pool of circular plasmids to increase each plasmid's signal?

Hello, Here is my experiment: I transfect in a plasmid that has been cut once (a linear piece of dsDNA) into human cultured cells. I wait 48hrs to allow the cells to "repair" the plasmid making...

10 February 2021 6,090 3 View

Recommendations for IL-10 Receptor staining?

11 October 2020 9,412 3 View

What is appropriate to use as my affiliation for a manuscript after finishing graduate school?

I would like to publish my graduate (M.S.) research, which is wildlife/conservation-related, and which I conducted at a U.S. university. It's been a few years since I finished graduate school. I...

14 September 2020 6,153 19 View

Do you mind answering a quick survey ? One minute only ?

Hi How are you ? languages are important in our world.. Mostly for you, if you need us to proofreading your articles. You need good translators to do so.. I set up my translation agency by the...

23 May 2020 1,323 3 View

What marker can I use to analyze dendritic spine maturity on a tissue section?

I would like to quantify the number of dendritic spines in all the maturation stages by using a coronal section of a mouse brain. I will be looking more specifically in the hippocampus.

30 September 2019 7,816 5 View

How do I write a PDB file with custom non-atom particles for a coarse grain model for molecular dynamics simulations (on GROMACS)?

I am trying to write custom particles to a PDB file for coarse grain molecular dynamic simulations in GROMACS. In particular, I am trying to model RNA nucleotides using a handful of beads per...

05 July 2018 7,785 2 View

Uninvited guest in intestinal organoids?

Please excuse the poor photograph. This very large body has appeared in my mouse intestinal organoid culture. To the right (out of focus) is my budding organoid, and in the centre is this very...

14 February 2017 6,791 2 View

What causes huge black dots on (nitrocellulose) membranes?

Dear all, I transferred proteins labeled with a fluorescent dye from (a part of) a 2D SDS-PAGE gel to a nitrocellulose membrane. After scanning the nitrocellulose membrane to check whether the...

27 October 2016 2,816 3 View

Is silver citrate hydrate and/or silver acetylacetonate soluble in ethanol?

For a synthesis I need a silver derivative that is soluble in ethanol. Does anyone have any experience on how well silver citrate hydrate or silver acetylacetonate dissolve in ethanol? It is...

28 July 2016 1,376 3 View

What is the best method to avoid contamination between samples that need to be homogenized prior to DNA extraction?

We have many samples to be homogenized and the material need to be washed between samples to avoid DNA contamination between them. It is hard to effectively bleach all the materials. I did some...

01 May 2016 443 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

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03 March 2021 5,360 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View