Hi!
We are struggling to perform something which I had hoped would be quite standard. CD3-staining on mouse spinal cord from EAE mice (should be plenty of T-cells). The tissues were PFA-perfused, sucrose protected and frozen.
Most nice looking stainings that I can find in the literature are done on paraffin-embedded tissue. Our samples are already collected and quite valuable so I would prefer to use it as is.
Anyone with a good protocol that works with this particular setup? Please specify which antibody you are using.
Very thankful for any help.
/Harald
Enrico Marani
If your Ab is made by glutaraldehyde coupling, try to find an antibody based on formaldehyde coupling.
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