Harald Lund

3 Questions 9 Answers 0 Followers

Questions related from Harald Lund

CD3-staining on PFA-perfused and frozen mouse CNS tissue?

Hi! We are struggling to perform something which I had hoped would be quite standard. CD3-staining on mouse spinal cord from EAE mice (should be plenty of T-cells). The tissues were PFA-perfused,...

02 February 2017 3,975 1 View

How do I best homogenize mouse hippocampus for analysis of aggregated/phosphorylated tau?

I have dissected out mouse hippocampus and want to homogenize it for analysis of phosphorylated and aggregated tau. We have dounce homogenizers but they feel too big for such small tissues. We...

12 December 2014 960 8 View

How many microglial cells do I need to be able to do qPCR?

I am planning to sort out microglial cells from mouse brains after percoll separation either by FACS or by using CD11b-magnetic beads. How many cells can I expect from one mouse and how many...

11 November 2013 997 7 View