Does anyone had used the protein nitrosylation kit from Cayman with mouse brain samples? I’m looking for a protocol to process/extract protein from my tissue that is suitable to use with this kit.
We had some phenol and guanethidine contamination in our RNA samples, providing an inadequate A260/230 ratio. Any tips on how can I overcome that? Actually, how could I reprocess my diluted RNA to...
09 October 2019 2,031 3 View
I'm new to Flow cytometry and was wondering if there is a way to find out which are your fluorophores, when the samples are not labeled, just looking to the graphs. I had some problem with the...
09 October 2015 6,782 4 View
I know that there is a demonstration of increased intracellular calcium in astrocytes induced by TNF-a, but I couldn't find evidence showing that this could occur in neurons.
02 March 2013 5,018 7 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Do You Know Untreated TMJ Affects Your Brain And Entire Body?
04 August 2024 6,282 1 View