Does anyone had used the protein nitrosylation kit from Cayman with mouse brain samples? I’m looking for a protocol to process/extract protein from my tissue that is suitable to use with this kit.
We had some phenol and guanethidine contamination in our RNA samples, providing an inadequate A260/230 ratio. Any tips on how can I overcome that? Actually, how could I reprocess my diluted RNA to...
09 October 2019 1,952 3 View
I'm new to Flow cytometry and was wondering if there is a way to find out which are your fluorophores, when the samples are not labeled, just looking to the graphs. I had some problem with the...
09 October 2015 6,705 4 View
I know that there is a demonstration of increased intracellular calcium in astrocytes induced by TNF-a, but I couldn't find evidence showing that this could occur in neurons.
02 March 2013 4,939 7 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
I have selected brain tumor images ...but now found that already lots of research done n this topic.
03 March 2021 5,774 3 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...
02 March 2021 2,735 2 View
I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.
01 March 2021 4,306 1 View
There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...
01 March 2021 831 2 View
I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...
01 March 2021 7,675 3 View
I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...
01 March 2021 8,079 2 View
The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.
28 February 2021 9,464 2 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View