How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Can anyone help for the content validity of my psychological test?

Good day! I am in the midst of completing my test development requirement under the Psychological Assessment subject. I was wondering if anyone can help me reach experts or psychology graduates...

14 January 2021 1,536 4 View

Can anybody here suggest theories of prudence?

13 January 2021 4,962 2 View

Where to find ReaxFF parameters for Si-H systems?

The 2003 van Duin et al. paper has Si-O-H systems, but is there a source for parameters without those pesky oxygen atoms? for Six-Hy only?

08 December 2019 4,106 2 View

Is there any Open Source Simulation Software including a harmonic Solver for low frequency electromagnetic Fields including Eddy Current effects?

here I am mainly looking for FEM Software I know that some commercial software like ANSYS, ANSYS Maxwell, Abaqus, COMSOL can do this, but doing a quick research I could not find any...

22 May 2019 8,633 5 View

Does anyone know a freely usable intelligence or reasoning test?

I'm planning to conduct an online study, in which participants have to complete an intelligence test. Because I'm unsure whether I can use traditional IQ tests such as the APM without copyright...

17 May 2015 3,119 7 View

Can I use a polyclonal antibody for quantification?

I want to quantify eGFP in cell lysate via Western Blot. Are there any disadvantages of polyclonal antibodies in comparison to monoclonal antibodies, besides possible cross reactions?

13 November 2012 3,247 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

How can I separate Gertrud Pfister's profile and the profile of Gerald Gems?

we are co-authors of several publications. But I am the only and single author of most of my publications which are based on my own research. How can I separate the profiles?

21 February 2021 4,582 3 View

Why use both bead beating and enzymes (lysostaphin and lysozyme) for DNA extraction from sputum samples?

Hello, I have read in a few publications that to extract total DNA from sputum samples, both bead beating and enzymes are used to disrupt the cells. Bead beating is supposed to be efficient in...

18 February 2021 3,360 2 View

Change of First name?

In some publications you refer Deivid Chica (with Barranco) but the true first name is Maria Dolores Chica , in spanish Ma. Dolores Chica. Before I answer yes is an author Deivid Chica, but...

18 February 2021 5,004 3 View

How should I compute a new variable with z-scores?

I would like to create a new variable using 4 different measures: hIndex (range: 1-47), # of publications (range: 0-111), and 2 other measures (range: 1-7). I changed all of these variables into...

09 February 2021 4,866 3 View

Do you know any reporting tool, guidelines for credibility assessment tool used for studies/publications with Artificial Intelligence?

I want to conduct a Systematic Review and assess the quality of methods used/proposed by researchers. I found out that scientists often utilize QUADAS for that...

05 February 2021 8,743 5 View

How do Research Gate proceed to integrate in the reference data publications and citations the recently published journals ?

How do Research Gate proceed to integrate in the reference data for publications and citations the recently published journals - for instance for the last months of 2020 and for instance Intl J...

02 February 2021 9,336 7 View

How to add journals not listed on Research Gate ?

I have two publications one listed in the web of science master list " European Online Journal of Natural and Social Sciences " ISSN: 1805-3602 and Second " Journal of Managerial Sciences"...

26 January 2021 7,158 7 View

Can you recommend a good book on analytical chemistry for non-chemists?

Dear colleagues, I am asking for you recommendation: I need a book on analytical chemistry in environmental sciences. As a non-chemist (a biologist) I have some basic chemistry knowledge but not...

25 January 2021 8,486 8 View