I had tried lot of times for culturing of Cyanobacteria in modified Allen and Nelson Media. It survived only one week. Then it begin to die. Would you suggest any protocol for culturing of Trichodesmium?
HI! Jabir ; You can grow Trichodesmium in ASN3 medium with are without nitrogen source.
Trichodesmium Icultures were grown in YBCII
medium 12: 12 light/dark cycle at ,80 mmol
photons m22 s21 white light . Diluted batch
cultures were grown in sterile square 1 L Nalgene bottles as single
filaments with gentle bubbling, sufficient for preventing aggregates
formation without harming the integrity of the filaments
Look on Berman-Frank ppares
HI! Jabir you can try ASN3 medium with or without any nitrogen source.
regards Tarkeshwar
Hi, I think you may get some information from these papers
• Bell PRF, Uwins PJR, Elmetri I, Phillips JA, Fu F, Yago AJE (2005) Laboratory culture studies of Trichodesmium isolated from the Great Barrier Reef Lagoon, Australia. Hydrobiology 532: 9–21
• Ohki, K. and Y. Fujita: Laboratory culture of the pelagic blue-green alga Trichodesmium thiebautii: conditions for unialgal culture. Mar. Ecol. Prog. Ser. 7, 185–190 (1982)
• Ohki, K., J.G. Rueter and Y. Fujita. 1986. Cultures of the pelagic cyano- phytes Trichodesmium erythraeum and Trichodesmium thiebautii in synthetic medium. Mar. Biol. 91: 9–14.
• Ramarnurthy, V. D. (1972). Procedures adopted for the laboratory cultivation of Trichodesmium erythraeum. Mar. Biol. 14: 232-234
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