Can you suggest alternate chemical for potassium tetraborate k2b4o7 which is essential for chitinase assay?

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What are the Hypotheses can i frame for the topic " Social media as a Source of Information to Engineering Students"?

i am planning to do research on the topic " Social media as a Source of Information to Engineering Students" for this please share valuable thoughts.. which type of Hypotheses can suitable for...

27 October 2018 8,864 3 View

Can okra plant be propagated by stem cuttings?

Dear All, I have identified a putative male sterile line in okra(Abelmoschus esculentus- through wide hybridization) (confirmed sterility with crossing/ selfing/ pollen studies). In order to...

10 October 2017 7,706 3 View

Clone having repetitive sequence insert and its discripancy upon scale up?

I have cloned my 3.3Kb insert having repetitive sequence in yeast expression vector, by directional cloning in E.coli. Positive Clone of 8.7Kb was confirmed by restriction analysis. While the same...

26 January 2016 4,477 2 View

How can I implement a indirect matrix converter controller?

electrical engineering

11 February 2015 6,989 3 View

What are the strong reduction agents to remove the epoxy(=O) groups and -COOH groups?

what are the different reducing methods and reduction chemicals...

24 August 2014 2,993 3 View

Comparison of Nano drop and RNA integrity number (RIN) data?

I have isolated total RNA from mammalian cell culture samples. I used a Qiagen Kit. I have checked the conc and quality of RNA by Nanodrop. Ratio of 260/280 was between 2.01 and 2.09, and 260/230...

21 August 2013 4,011 16 View

mRNA level and its comparison to protein level.

I am measuring a gene which is involved in differentiation of a cell. During differentiation its level is high, compared to undifferentiated state. Upon terminal differentiation of cell, mRNA...

20 June 2013 2,588 2 View

suggestions for using alternate pcr method for race

hello friends! any one suggest me alternate pcr method for plant cloning and expression instead of using race..

25 July 2011 3,681 1 View

Chromosomal counting in okra species?

I have been trying to prepare root tips for chromosome counting studies in okra (Abelmoschus), but every time I am failing to get stained root tips with Feulgen stain. The root tip portion is not...

01 January 1970 5,105 4 View

Need project/publication details regarding IoT application in disaster management

I want to pursue my research in IoT domain. Can anyone share details regarding implementation of IoT in disaster management? I wanted the link of project details or published articles in the...

01 January 1970 9,934 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View