I would like to conduct a research on the Impact of Facebook usage to the Character Building of Grade 10. What question/s do you suggest?

The study will be conducted to grade 10 students in the Philippines

17 July 2019 2,788 3 View

How extract information of molecular chains from .xtc file (gromacs)?

I barely discover Gromacs and I have to use it to study trajectory results, so I wanted to know if it was possible from an xtc file to generate several xtc files, one for each chain of the...

25 March 2019 2,945 5 View

Help figuring out if certain murine cancer cell lines express specific cancer antigens. Is there an easy way?

I've been tasked with figuring out if certain murine cancer cell lines express specific cancer antigens we would like to target. For instance, does the 4T1 breast cancer tumor model express WT-1?...

30 April 2017 5,588 1 View

No title

23 October 2016 5,023 3 View

Due to the drastic price reduction of PV modules, do you think high concentration PV systems are still cost-effective?

The price of PVmodule in about 15 years is 1/10, whereas the price of PV systems is halved [1], it implies that the impact of PV modules on the cost of the whole systems is decreasing more and...

08 November 2013 5,020 4 View

Does anyone have experience with XLSTAT Tukey test (one-way ANOVA results)?

Any reason why there is inconsistency between significant table (contrast) and grouping table (category) in the Tukey test?

02 September 2013 1,666 8 View

Is the reactivity of a functional group affected by the type of molecule that carries it?

Is there a possibility to predict or estimate the reactivity of an epoxy functional group, by means of a comparison whether it is bond to aromatic or aliphatic molecules, respectively?

30 August 2013 8,831 10 View

Protoplast storage conditions

Does anyone have an idea if maize protoplasts could be stored? If yes, do you know the conditions and the efficiency of the protoplast after storage?

11 July 2013 8,671 1 View

Opinions on the morphological changes of these cells.

These cells are from an osteosarcoma cell line. They have been treated with a chemotherapeutic agent for 72 hours in a Co2 incubator at a concentration which has been found to induce apoptosis and...

31 January 2013 8,336 26 View

How can I get an even spread of cells in a 24 well plate?

Does anyone else have an issue with evenly spreading cells in a 24 well plate? No matter how careful I am, the cells (MG-63) huddle mostly in the middle of the well in the outside wells of the...

20 November 2012 3,913 31 View

How can the use of Jolly Phonics improves the quality of reading, speaking and writing in rural disadvantaged communities in Papua New Guinea?

Jolly Phonics reading program is an effective way to teach early reading to young learners. How can lectures at the university prepare early childhood student teachers to teach Jolly Phonics to...

03 March 2021 3,731 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

No title

22 February 2021 9,932 11 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View