This is from the oncomine comprehensive assay v3 protocol. Does "gDNA (10 ng, ≥0.67 ng/μL) " mean that the concentration range of gDNA should be from 10 ng to ≥0.67 ng/μL, and If I already have 10 ng/ul, do I set up a reaction without Nuclease-free Water?
Mikheil Jangavadze
I think you mast have ≥0.67 ng/μL concentration in one well. You can get it from adding 10ng gDNA in 15 ul water . (10/15=0.67).
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