Can you express sgRNA with a none U6/ U3 promoter in an easily cloned vector?

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Automated software for grooming and rearing behavior in mice?

Hi all, I have a set of open field test in which i want to detect grooming, supported rearing and unsupported rearing behavior. Ethovision XT was somewhat OK for the grooming but couldn't...

31 December 2019 890 1 View

How to manually score a behavioral test for free?

Hi all! I have performed a behavioral test (visual cliff) and i want to classify the mouse location in 3 different parts of the arena, I want to be able to extract cumulative time, latency to...

31 December 2019 6,988 2 View

RNA extraction with phenol chloroform, leaving in -20c?

Hi all, In our lab we have a lot of experience extracting RNA using Trizol. I know (and have done this) that after taking the top phase and adding isopropanol you can leave the sample in -20c to...

11 December 2019 2,785 3 View

Control for lentiguide puro?

Hi all, Do you think that inserting 20nt of T (poly T) instead of the guide RNA will be a good control? as poly T is a termination signal for U6. Thanks!

10 November 2018 3,404 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Gidi Karmon

Thanks! I will try it. Still, if anyone has more ideas ( just in case) I would greatly appreciate it!

Di Xia

Hi, Gidi, I am facing the same issue. One possibility is to do sgRNA IVT or to use Chemical synthesised guide RNA. A few companies are making that. The best ones are from IDT (crRNA + tracrRNA) or Synthego (full length sgRNA).