Gidi Karmon

5 Questions 3 Answers 0 Followers

Questions related from Gidi Karmon

How to manually score a behavioral test for free?

Hi all! I have performed a behavioral test (visual cliff) and i want to classify the mouse location in 3 different parts of the arena, I want to be able to extract cumulative time, latency to...

01 January 2020 7,101 2 View

Automated software for grooming and rearing behavior in mice?

Hi all, I have a set of open field test in which i want to detect grooming, supported rearing and unsupported rearing behavior. Ethovision XT was somewhat OK for the grooming but couldn't...

01 January 2020 960 1 View

RNA extraction with phenol chloroform, leaving in -20c?

Hi all, In our lab we have a lot of experience extracting RNA using Trizol. I know (and have done this) that after taking the top phase and adding isopropanol you can leave the sample in -20c to...

12 December 2019 2,849 3 View

Control for lentiguide puro?

Hi all, Do you think that inserting 20nt of T (poly T) instead of the guide RNA will be a good control? as poly T is a termination signal for U6. Thanks!

11 November 2018 3,465 2 View

Can you express sgRNA with a none U6/ U3 promoter in an easily cloned vector?

I need to design an sgRNA that contains TTTT thus making expression with RNA pol III impossible. It will need to be expressed in mammalian cells. Any help will be extremely welcome (please refer...

01 January 2018 6,244 2 View