Can you answer some questions about mental fatigue in sports?

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How to determine fluorescence quantum yield for photoswitchable/photochromic compounds?

How to determine FQY for photoswitchable compounds? I found most related publications didnt mention, or just said that they used "Absolute quantum yield spectrometer." I think such technique...

31 October 2020 8,002 5 View

Hi everyone, how can we remove the snow-covered areas in global scale?

We obtained the global results using remote sensing reanalysis dataset. But the snow-coverd areas should be removed (like the blank area in the picture below, sources: Purdy et al., 2016). Does...

20 September 2020 3,463 5 View

How to add a scale bar for images acquired from Molecular Device Imagexpress pico?

I saved some images when I scanned certain area using imagexpress pico. But don't know how to add scale bar either in system or image J. I am wondering if anybody can provide some help on that?

17 September 2020 8,068 4 View

How should I design the N-terminal VC plasmid of BiFC assay?

I'm going to detect protein-protein interaction with BiFC assays and my target proteins are ER retention with KDEL motif. so I want to fuse VC to the N-terminal of my protein. My question is if...

22 July 2020 3,456 1 View

How to grow more BL21 auxothrophic strain cells?

Dear all, I have just purchased a BL21 auxothrophic strain (RF12) from Addgene. The cells come in the form of agar stab. I intend to grow more cells which will be ready to used for protein...

11 May 2020 8,384 3 View

Roche has stopped its Universal Probe primer design service, does anyone know where or how we can design primers to fit Universal Probe Library?

Roche announced the Universal Probe primer design service will be stop in the end of 2020, but the primer design website has already been shutted...

10 February 2020 4,745 2 View

How do we make multi Fatsa files?

Hi, previously I had used MUMer to run but the input are only able to upload 1 Fasta file. When I test run a reference and the sample it work great but when I combine my sample 1 and sample 2 data...

25 July 2019 3,681 1 View

Does anyone know of a cancer cell line with c-myc gene amplified?

Breast cancer cells preferred, but other cell lines work too. Thank you.

06 May 2019 5,018 4 View

Is it possible and beneficial to learn probability distribution of input data when we apply machine learning methods like artificial neural network?

Hello, everyone! In recent time I am learning an online course called "learning from data". The lecturer showed the below learning diagram you can find from this website:...

29 October 2018 2,510 3 View

Preparation of polyimide solution?

As i am working on liquid crystal material and till now we are using commercial samples which already had the coating. But now i need to device the cell with modified substrate for my current...

26 August 2018 1,779 3 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

Mixed ANOVA - help?

I'm currently working on a piece which asks to conduct an ANOVA - looking at the effect of sporting group (2 levels), and exercise trials (two trials with six levels). I've computed this all...

27 February 2021 9,626 4 View

Multinomial probabilities and confidence intervals - obtaining the CI of the reference category?

Hi, Some background to my question: I have fitted 1st order multinomial Markov models with covariates. Since all probabiilties (for each row of the transition matrix) sum to 1, I estimate only...

22 February 2021 2,541 4 View

Alternative to mediation analysis?

I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....

21 February 2021 8,609 1 View

Open Source IoT Simulators supported by Machine Learning?

Dear Research Gaters, Let me first add context to the question. I am looking to simulate the waste management system and resources of a city. The hardware devices I need to simulate include,...

21 February 2021 2,333 4 View

How COVID-19 has affected mental health?

The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.

21 February 2021 4,733 19 View

How to correctly choose the number of solution intervals when preforming harmonic analysis in ANSYS?

Good day, I design the rail-wheel geometry and the harmonic frequency ranges between 20-10kHz with 500Hz solution intervals and the results makes sense. Most of the time on the related literatures...

19 February 2021 3,190 3 View

What is the original citation for the commonly used activity factors for converting basal metabolic rate to total caloric need?

I see the same activity factor (multipliers) in research papers and websites, but I cannot find where they come from. Does anyone know where these originated? I am looking for an original...

18 February 2021 7,635 2 View

Are there any exception like work hardening in metals elastic limit due to dynamic loading?

The samples made out of spring steel are taken from same lot. The specimens were shaped following heating to forging temperature - Forming- Hardening -Tempering methods. After storage of almost 6...

11 February 2021 952 6 View