How would you mechanically support the the film? If you could intercept the incident light of the photo spectrometer by the film material it is then okay. All what you need is to subject the material to the light produced by the spectrophotometer. But how would make this without suitable substrate?
NOTICE: i added the last question after the comment of Dr. U,Dreher
As it was discussed above, if your sample is mechanically stable, it is better to do this without glass substrate. The glass substrate sometimes creates noise in the spectrum.
The most important specs of the the spectrophotometer are the wavelength range of its light source and emitted intensity of light in a selected wave length range. So, you have to check whether the specrophotometer at hand has the specifications for successful measurement.
yes sir it is from 200 to 800nm. But here usually students use liquid samples in cuvette not thin film I read a paper in which they used uv/vis spectrophotometer to determine the thin films but no specification was mentioned for the instrument therefore I want to confirm that my thin film could be studied directly by the usual UV/Vis spectrophotometer or not