Dear Dr Mohammadabadi,

can you describe clearly your question? Do you want to use of bovine and or ovine data for laboratory animals models, like Rats?

There  is a good body of literature for Rats and herbal extracts!

On the other hand, As you know better than me, feed digestibility is different between ruminants and the others. and its obvious that "ruminants  are mammals that are able to acquire nutrients from plant-based food by fermenting it in a specialized stomach prior to digestion, principally through microbial actions."then If I understand your question, I suggest you gather a good literature in laboratory animals and or you can design a pre-experimental study before your main study.

Regards,

Mehdi 

salam

Thank you Dr Mehdi.I don't want to use on laboratory animals? I want to use especial herb in sheep ration that influence reproduction system, but there is rare information about amount should be used. so I read in papers that herbs used on based of body weight? 

Dear Tahereh

First the dose is depends on the rout of administration, for example if it is per os (orally), the dose is so different in ruminant than single stomach animals and/or laboratory animals. Second: you can use dose response curve to calculate the dose in ruminant, for example: if you need to calculate the dose of X compound or extract on male or female reproductive performance in vivo, choose five different doses (x, 5x, 10x, 20x and 50 or 100x) or logarithmic gradient (x, 10x, 100x, 1000x, 10000x), give these doses to the animals (5 animals per each dose) for such period, obtain blood samples and assess (for example) FSH level (if your research on females) or testosterone level (if your research on males). Then you can calculate the effective dose using statistical procedure (dose response curve).

Kind regards 

Thank you Deat Dr Jabbar. sorry i cant understand about x, 5x, ...

i mean what about x?

Dear Tahereh

x means the starting dose for example 50 mg/kg, so 5x is 250 mg/kg, 10x equal to 500 mg/kg, 100x equal to 5000 mg/kg

Kind regards

Hi dear Tahereh, 

Im agree with dr jabbar idea approximately, but at first you must select the low dosage of your materials, and then select one of the two ways. With an eye to this point that high dosages of herbal plants not only have not ammeliorative effects but also have deleterious effects in reproductive indices. On the other hand, i dont know, why Dr jabbar conveyed these parameters such as FSH for female and T for males., in this issue you must focuse very well, for example, in the three articles that we published recently we stablished that T concentration for male is not enough then its better that we calculate T/E2 ratio.

I think if you couldnt find your herbal plants exactly, you can find the dosages for another similar (characteristic)  herbals, for example in 2013, I wanted to determin our dosages for Sage (salvia officinalis) then i could not find their dosages, but i found a manuscript which is published by Ahmed and  abdollah,... for Rosmary., then i decieded to had a pre-test like their dosages that our results became great,fortunately.

I hope all the best in your research.

sorry, thesedays we are in vacation for the first days of May, I wrote this text by my cellphone to you.

Mehdi

Dear Ommati

Dear Tahereh

Many thanks to you for your comment. I would like to mention that I choose these parameters just for examples. The researcher can choose any suitable indicative parameter matched to her goal.

Accept my best wishes

Dear Dr Jabbar

Thank you for your reply. but in this sentences "x means the starting dose for example 50 mg/kg, so 5x is 250 mg/kg, 10x equal to 500 mg/kg, 100x equal to 5000 mg/kg"

if can we start dose, less than 50?

mg/kg, it means kg/ BW?

Dear Mehdi

Thank you so much. can send me this paper?

......by Ahmed and  abdollah,... for Rosmary.

Dear Dr Jabbar

Thank you for your reply. but in this sentences "x means the starting dose for example 50 mg/kg, so 5x is 250 mg/kg, 10x equal to 500 mg/kg, 100x equal to 5000 mg/kg"

if can we start dose, less than 50?

mg/kg, it means kg/ BW?

Regards

Dear

If you start with 10 mg/kg body weight, so it will be x. 5x will be 50 mg/kg bw, 10x will be 100 mg/kg bw, 50x will be 500 mg/ kg, you can increase the duplication more and more no problem. from your results you can exclude the result of the dose that causes side effect. from your results you can do dose response curve (statistically), and therefor the statistic will give you the effective dose. also I would like to mention that statistic may give you (for example) the effective dose equal to 11.5x, so the real dose is 115 mg/kg bw.

If you need more details, just write me to send you complete example by email.

Best wishes

Dear Dr

these amounts is belong to plant extract? Now I going to use plant powder? so how to change these amounts?

sorrry to bother you.

Dear Tahereh,

Please download this article and read it:

https://www.researchgate.net/publication/273873114_Seminal_characteristics_sperm_fatty_acids_and_blood_biochemical_attributes_in_breeder_roosters_orally_administered_with_sage_Salvia_officinalis_extract?_iepl%5BviewId%5D=jkvj3Ga1Hde88AN29CCEyimt&_iepl%5Bcontexts%5D%5B0%5D=prfhpi&_iepl%5BtargetEntityId%5D=PB%3A273873114&_iepl%5BinteractionType%5D=publicationTitle

Best wishes,

Mehdi

Article Seminal characteristics, sperm fatty acids, and blood bioche...

than you very much

Dear Tahereh,

Please have a look to the following article:

Plants Toxic to Farm and Companion Animals

Chapter · January 2017

DOI: 10.1007/978-94-007-6464-4_23 ·

In book: Plant Toxins, pp.107-134

Good luck,

Mehdi