Anyone knows how to extract RNA from samples with high starch content (wheat) by using RNeasy Plant Minikit?

I am using 74904 to extract RNA from wheat seed and I have tried both of the lysis buffer. As it is mentioned it protocol, buffer RLT is the lysis buffer of choice but it may cause...

14 October 2020 9,409 7 View

How long does it take for MCF-7 cancer cells to adhere to the microfluidic surface? Is 24 hours enough?

Hello everyone. We have cultured MCF-7 cancer cells in microfluidics several times and incubated them for 24 hours, but as the nanomaterial passed through the cells, the cells moved and no cell...

05 September 2020 7,292 3 View

How can I measure Superoxide dismutase (SOD)?

Hi everyone, SOD measurement need to making assay buffer (Na-PO4 buffer+ NBT, L.Methionine and Riboflavin). I prepare this buffer under dark condition but unfortunately after about 10 -15 min its...

10 September 2018 937 5 View

How can I make DMEM F12 with 30mM glucose concentration for induction of stress oxidative in HUVEC cells?

Hi dear researchers, DMEM F12 glucose concentration is 17.5mM, I added D_glucose in basal cell culture until reached 30mM, but HUVEC cells had better grown than control group after 48 hours. I...

06 April 2018 8,650 1 View

Clustering network vehiculer in ns2?

I need to compare my work with the Reservation-Based (RB)code that described in the "Offloading Floating Car data" article. Can anyone give me the its code? I work with ns2 simulator

06 September 2017 3,631 1 View

What is the longest lifetime reported for electron and holes in perovskite solar cells to date?

When these materials get excited with light, electron and holes are generated but it is important for them to hang arround for a while before they recombine so that more photocurrent will be...

26 August 2017 9,161 11 View

What is the density of poly vinyl alcohol (SB220)?

Density of PVA (SB220)

06 February 2017 8,921 5 View

Why the type of organic solvent can affect particle size of nanoparticles in film hydration method?

For example dichloromethane and chloroform

11 November 2016 1,428 1 View

How can i detect fire using SAR (RADARSAT-2) backscatter and optical data?

I would detect fire, insect and deforestation (logging and building) disturbance using SAR backscatter and Optical data for Hyrcnian forest in the Iran.

02 October 2016 2,129 4 View

How to prevent/reduce hysteresis effect in dye sensitized solar cells (DSSCs) / perovskite solar cells?

I have read that one of the major challenges in solar cells applications is reducing Hysteresis. First off, can anyone give an explanation of this phenomenon? Secondly, how to face this...

27 July 2016 8,620 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View