In my previous experiments, i used MAP1LC3B (LC3BII) antibody to map autophagy. I want to check the same using MAP1LC3A (LC3AII) antibody. I am aware of their subcellular distinct expression. Can we use the antibody to monitor autophagy?
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Hie everyone The treatment used in my study is showing increase in Beclin-1 expression and Lc3b-II turnover in M.bovis infected macrophages. The same treatment led to reduced expression of p62...
02 February 2018 1,660 4 View
Hie I am working on a peptide which is inducing autophagy in THP1 derived macrophages and thus clearing mycobacterial infection. I found upon peptide treatment, the M.bovis infected macrophages...
30 January 2018 3,698 4 View
31 December 2017 3,297 4 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...
01 August 2024 476 2 View
I have a question about human macrophages cell surface markers.I want to stain the unpolarized human macrophages for flow cytometric sorting without permeabilizing them. It is because I need to...
29 July 2024 236 2 View
I have three linkage maps that needs to be integrated to create a consensus map for QTL mapping.
25 July 2024 6,505 2 View
Does anyone know of any published articles that provide guidance on using TA with multiple team members? I am aware of one (see below), however, there are some disagreements in terms of some of...
23 July 2024 7,819 5 View
Hello researchers, I have sequenced the A549 cell line using RNA sequencing approach and then I have it against the human genome reference sequence. Would I expect a 100% mapping percent? I got...
20 July 2024 9,705 0 View
Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...
19 July 2024 2,155 0 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View
Recently, I tried to differentiate THP1 into M2 macrophage using IL4 and IL13 (purchased from R&D). This is my protocol. THP1 was seeded into 6-well plate (10E6 cells/well). Incubate with PMA...
15 July 2024 5,153 2 View
Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...
15 July 2024 4,615 1 View