CP ChromoSelect Agar is an ideal growth media. It contains only vegetable peptones and, together with yeast extract, it is an excellent source of nitrogen, carbon, amino acids and vitamin B complex. Sucrose acts as the fermentable carbohydrate and reducing agents lower the redox potential of the media. Diverse salts provide the required ions for enzymatic reactions. Buffering agents stabilize the pH within the ideal growth range. Inhibitors D-cycloserine and polymyxin B give the medium its selectivity, while further selectivity is achieved by incubation under anaerobic conditions at 44 °C. Various promoters and substrates protect injured cells to improve recovery rate and enhance growth. The chromogenic enzyme substrates in the CP ChromoSelect Agar provide the differentiation, particularly for C. perfringens. A negative indol reaction (Kovac’s Reagent) confirms the presence of C. perfringens.Clostridium perfringens is found in undercooked or improperly sterilized canned foods (germination of endospores) and in water (surface water). The natural contamination source is human and animal faeces transmitted into food products primarily by water. C. perfringens produces an extensive range of invasins and exotoxins. The enterotoxins cause the undesirable, predominantly meat-associated, food poisoning, as well as wound and surgical infections that lead to gas gangrene.

Sir,

Thanks for your thoughtful reply. I wanted to know about its use in enumeration from fecal samples.

#patrick

Even I believe so. The method they have recommended is a filter membrane based tachnique. But I believe that can't be used for stool samples. Homogenised samples need to be spread over the plate.what do you suggest?