Md Iftikar Hussain

5 Questions 17 Answers 0 Followers

Questions related from Md Iftikar Hussain

Why is there no proper separation of DNA ladder?

Recently I have been using a High range DNA ladder ranging from 48kb to 10kb with manufactures instruction. But I am not able get a good separation.Bands are found to be staked at one end of the...

27 April 2015 3,630 5 View

Can we use CP chromo select agar for enumeration of c.perfringens from stool samples?

CP chromo select agar has been used to determine c.perfringens load in water sample. I want to use it for stool samples. Please suggest.

03 March 2015 2,926 3 View

Can agar cause problems in Glycerol stock?

Can Agar cause problem in glycerol stock? I have this specific doubt as I am not getting good bacterial growth upon revival of Clostridium perfringens glycerol stocks.

02 September 2014 6,697 9 View

Is it common to encounter non hemolytic perfringen colonies after one sub-culturing or glycerol preservation?

I have encountered some of the C.perfingens strains without hemolytic activity. I have also noticed that the colonies have distinct morphology over the hemolytic colonies. Some people have advised...

26 July 2014 9,080 2 View

How to perform antimicrobial disk susceptibility tests?

I am confused regarding the standards I should look for while doing antimicrobial disk test for clostridium perfringens. Diameters are not available! I have one copy of CLSI guidelines, but its...

19 March 2014 4,856 4 View