Can we use Actin/GAPDH as a loding control in Methylation and Unmethylation specific PCR?

Vasudevarao Penugurti @Vasudevarao-Penugurti-2

29 May 2021 0 4K Report

We are performing Methylation and Unmethylation specific PCR using primers for few regions in a gene of interest. We have a doubt that can we use actin/GAPDH as a loading control.

If anyone doing this kind of experiment, kindly suggest the loading control for this experiment.

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