We are trying to produce the mouse hybridomas . we want to skip the feeder cell in the process. Instead we want to add the HFSC as a supplement in the medium. which will be more correct way to produce the hybridomas?
Hello Balkrishna VASANTRAO Hatwalkar
Yes, you can. Feeder cells supply growth factors that promote growth of hybridoma cells. However, there are commercially available preparations containing growth factors that can support the growth of cells and can be used in lieu of feeder cells. So, HFCS can replace the feeder cells.
HFCS is composed of albumin, insulin, transferrin, cytokines, a cholesterol source, other defined organic and inorganic compounds, fetal calf serum and human proteins. It is used as a supplement to the normal culture medium to support the growth of B-cell hybridomas after fusion and during cloning.
You can also use HFCS to optimize the growth of hybridomas after thawing of cells stored in liquid nitrogen.
Best.
Hi Balkrishna VASANTRAO Hatwalkar,
I gave up the use of feeders (rat macrophages) to obtain hybridomas (during selection or cloning) after a few years of practice.
I simply coated 100μl of CFS HI in each well, 10 minutes at RT is enough.
By exploiting 5% of the fusion, I obtained several thousand hybridomas.
Respectfully
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