How to prepare OH- terminated Si Wafer?

Hi, Folks. I am working on the ALD project to deposit Al2O3 on the Si wafer from TMA and Water. I have struggled with the preparation of OH- terminated Si wafer. I have both Si(1 0 0) and...

08 February 2021 8,792 2 View

Real Time PCR: What is the justification for acceptable variance within 3 Ct? Is there any publication on this variance?

Hi all, I was not able to find any source with evidential justification for why the acceptable variance for internal control should be within 3 Ct and not other numbers of cycle of amplification....

17 December 2020 9,481 1 View

Methods for multivariate discrete-time time series clustering?

Can somebody suggest a popular method for clustering multivariate discrete-time time-series data ? My dataset is about water quality parameters taken in 6 stations by seasons in 5 years . Thank...

09 December 2020 6,933 12 View

Can you help complete a survey, if you know/ have used LPWAN technology in smart cities/transportation applications?

IoT/LPWAN User Survey Link: https://lnkd.in/dVdK-ea ----------------- Dear Sir/Madam, We are surveying the use of Low-Power Wide-Area Network (LPWAN) technologies (e.g., LoRa, SigFox, NB-IoT) for...

05 August 2020 9,001 3 View

How can I get the correct J-integral during crack propagation?

Hi. I'm learning GTN fracture mechanics in Abaqus/Explicit. But I can't get J-integral during crack propagation. Please, help me.

07 May 2020 6,589 3 View

I am conducting forest inventory in a tropical forest but I am not a botanist. Could anyone provide me the field guides for plant ID?

The inventory will focus on trees that has a diameter >5cm. Tropical Forest of Central America For example: Gentry's field guide to the families and genera of northern South America or any...

05 March 2020 865 3 View

Accuracy of land-use classification and uncertainties in land-use change assessment?

I am analyzing land-use change in 3 periods. For each period, one land-use map was derived from remote sensing technique along with accuracy of classification. I want to ask if there is any...

23 February 2020 8,063 5 View

How to design an exercise to enhance a psychological phenomenon such as self-compassion?

- What is the principle behind practicing/enhancing self-compassion? Or other words, how is self-compassion enhanced by practicing these exercises on Kristen Neff's website:...

04 November 2019 9,866 1 View

Has anyone conducted an ECCI experiment at high temperatures?

I know that ECCI is a powerful means of observing dislocations on the surface of the bulk material. However, unlike TEM, SEM, and EBSD, I have never seen the results of an ECCI experiment at high...

15 September 2019 5,660 1 View

How can I synthesis Amine and Chlorine including fragment?

Hi, All I trying to synthesis peptide with Amine (NH2) and Chlorine including fragment. I tried second times. 1) just left peptide with amine & 4X chlorine 3h 2) peptide with amine & 4X...

04 September 2019 6,534 4 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

How to measure pH in anaerobic digester?

I'm planning on doing research by using anaerobic digester with liquid substrate and want to see its pH changes periodically. Any suggestion on measuring the pH with maintaining its anaerobic...

09 February 2021 1,293 1 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

Can I use 500ng of DNA for restriction digestion?

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...

31 January 2021 729 6 View

After cutting bands having low molecular weight peptides obtained from SDS-PAGE,is it possible to elute these peptide(s) without digesting in trypsin?

Basically, I want to get the sequence of peptides. I assume, digesting these peptides from each band may give me a different sequence than the original through LC-MS analysis. If digestion doesn't...

30 January 2021 5,482 3 View

How to get the insoluble fraction of PK digestion for mitochondria?

I am trying to check for some proteins if they are located in the inner or outer mitochondria membrane after treating the cells with a neurotoxin. I am using PK to digest the mitochondria, but I...

27 January 2021 5,145 2 View