Can we ligate 2 different blunt end site ?

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I cultured microorganisms obtained from the soil on a medium. Can you identify what kind of microorganisms these are?

I cultured microorganisms obtained from the soil on a medium. Can you identify what kind of microorganisms these are? Is it possible that this microorganism is Stenotrophomonas panacihumi?

22 May 2024 875 4 View

What are the statistical approach for servant leadership in F&B?

To have multiple case study exploration among Malaysia restaurants that study the impact of servant leadership, what are the best statistical approaches for the case studies?

01 March 2024 2,737 1 View

Do solar winds impact the spread of communicable disease on the earth?

We know that solar winds had a 11 year circle and also human respiratory diseases also outbroke every 11-12 year. Is there ant connection between solar wind's strength and the pandemic...

18 February 2024 6,280 5 View

In low doping level, i want to know why N-type threshold voltage has negative charge?

Basically, we know that the N-channel MOSFET has a (+) threshold voltage. However, the graph has a value of (-) depending on the acceptor doping concentration in the substrate and oxide capture...

24 January 2024 7,817 0 View

How to calculate the osmolality of Hepes solution?

Here is an excerpt from Kollmann et al. (2020, J. Physiol.): "This ring was placed in a recording chamber continuously perfused with 37°C aerated Hepes solution containing (in mM) 136 NaCl, 10...

09 January 2024 4,766 2 View

Heme in protein - MD simulation?

Dear all, I am currently working on a protein simulation with a heme group in the protein. I am wondering whether anyone can tell me how I can set it up in GROMCAS with charmm forcefile, since...

17 December 2023 4,392 2 View

Is it possible to use SPSS PROCESS MACRO(Model 1) to test moderation with categorical IV + binary DV + continuous Moderator?

IV: categorical - (5 categories with 1 and 5 being the extreme indicators) DV: binary - (dummy coding has been done.) Mo: continuous 1) Is it possible to use SPSS PROCESS MACRO for moderation...

08 November 2023 4,421 1 View

Western blot sample prep problem?

Hello, I have a question about western blot sample prep. First, I am using adherent cells and RIPA buffer. After centrifuge at 4 degrees Celsius, small pieces are still found when collecting the...

01 November 2023 7,135 0 View

Does the small intestinal manometry measure the gas pressure or liquid pressure?

Small intestinal pressure is one of the GI motility indicators and its manometry is very advanced nowadays. I wonder if there is any liquid inside the lumen during manometry. I already know the...

28 October 2023 2,491 1 View

How to combine trajectory in GROMACS when I repeat the same simulation for three times?

Hi all, I am wondering how I can combine the trajectory in GROMACS. I used the coding 'gmx trjcat -s xx.tpr -f xx-1.xtc xx-2.xtc xx-3.xtc -o xxx-combined.xtc' and 'gmx trjcat -s xx.tpr -f...

16 September 2023 2,539 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

How to remove cell debris after tissue dissociation?

I'm facing issues with the tumor dissociation protocol for GBM. I use collagenase/dispase with DNase to dissociate my tumor sample. However, even after incubating it for 60mins, I do not get a...

22 July 2024 412 3 View

What additional processing or treatment options are available to enhance biogas plant digestate quality or value?

One has to understand about the enhancement procedures and treatment systems I can list around 12 procedures which are tried and tested and are proven so let us see how many in total the experts...

20 July 2024 3,392 2 View

How does the digestate compare to other organic amendments regarding quality and performance in different countries?

Experts are welcome to share their experience country-wise so that we can make a nice document listing all the country-wise experiences. ? How does the digestate compare to other organic...

18 July 2024 5,225 3 View

From different feedstocks based Biogas digesters what would be the standard NPK - Nutrient contents of the digestate, and how consistent is it?

One has to list down the details and understand that different feedstocks and the biogas digesters working on different feedstocks show different nutrient content value: But when it comes to...

18 July 2024 9,907 12 View

How can the economic value of digestate management, utilization, and regulation, can spark interesting discussions and explorations?

How can the economic value of digestate management, utilization, and regulation, can spark interesting discussions and explorations? so one can list down the details and we can discuss in length...

17 July 2024 3,255 4 View

Environmental Impact of the Organic Manure Production Unit?

Does anyone have any case studies on the Environmental Impact of Organic Manure Production Unit? Environmental Impact of a Organic Manure Production Unit Facility is what I studied but...

16 July 2024 5,400 6 View

What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized?

What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized - I have few of them listed and can be updated in this...

13 July 2024 2,431 2 View

Interested in Research Collaboration?

anyone looking for research collaboration on the anaerobic digestion slurry and digestate can contact me for further details. As my whatsapp number is +91 8317585217

11 July 2024 6,753 3 View