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Questions related from Hồng Minh Triết
The vector is linearized with 1 site EcoRV and dephosphorylated with CIP. The insert (~500bp) is digested and both end with Hinc II. Could we ligate the vector and insert together ?
22 March 2018 5,912 2 View
I plan to use Benchling for gRNA design because Zhanglab tool is getting too slow these days. Have anyone given a tried on the Benchling tool for gRNA design ?
20 March 2018 9,181 3 View
I extracted total RNA from HEK293 cell using Trizol, then I ran all my 3 samples on 1% TAE agarose gel for RNA quality check (Pls refer to the attached picture). But beside 28S and 18S bands...
19 December 2017 3,855 4 View
I am trying to treat the 21 days differentiated Caco2 monolayer with LPS at the concentration of 5µg/ml. During the differentiate stage (21 days), I feed the cells with DMEM 10%FBS, 1% NEAA, 1%...
05 July 2016 7,657 1 View
I try to clean data of massarray EpiTYPER using R script provided by GitHub (http://journal.frontiersin.org/article/10.3389/fgene.2015.00287/full). But I am stucking now because of this...
18 April 2016 257 1 View
For example : In this paper (http://www.jbc.org/content/274/5/2773.full). They studied the high-affinity peptide transporter using rat kidney brush-border membrane vesicles and oocytes expressing...
12 April 2016 4,232 1 View
