Can we extract DNA through boiling method from E.histolytica cysts?

10 August 2017 0 7K Report

I am currently working on DNA extraction from E.histolytica cysts. I used thermofisher DNA extraction kit but it didnt work.

Badges
Science topic

Similar topics
Phytochemistry
Extracts

More Dr Abdul Samad's questions See All

Are you looking for research collaboration ?

we have few papers ready for submission, and we need one co-author for each article who can pay article fee. Interested authors may text here or contact me on my following email id [email protected]

29 July 2024 6,626 0 View

Dear researchers. pl help how to plot jablonski energy level graph and magnetic hysteresis curve in origin?

through origin software

17 July 2024 4,991 0 View

How is artificial intelligence being utilized to enhance the diagnosis and treatment of sleep apnea?

AI has the potential to improve the management of sleep apnea by personalizing treatment, enhancing diagnostic accuracy, and advancing our understanding of the condition.

03 July 2024 9,393 2 View

How do pharmacokinetic and pharmacodynamic processes differ in children compared to adults, and how should drug dosages be adjusted accordingly?

Pharmacokinetic and pharmacodynamic differences, adjustments drug dosages in children to ensure safe and effective therapy.

21 June 2024 7,491 1 View

What are the good journals for Pharmacology?

I want to know about the journals that don't charge for the publication of an article and also the one that takes less time to process.

20 June 2024 9,211 0 View

Action research design is Quantitative Studies or Qualitative Studies?

14 June 2024 1,684 1 View

Offer or collaboration for book publications?

Dear Profs/ Scientist, if any one is interested or have plan to publish a good quality books/ book edited , I can contribute/help such type of research works . My research...

06 June 2024 5,932 2 View

How to draw SeDeM diagram for preformulation study of drug and excipients?

What is SeDeM expert tool? Is it in silico tool and how to draw the SeDeM diagram for preformulation study? Kindly suggest.

06 June 2024 4,408 0 View

Call of book chapter ?

CALL FOR BOOK CHAPTERS (No Publication Fee) Scrivener Publishing/wiley Dear Prof,Scientist/ experts, Greetings. We are editing the book entitled " FET Devices: Post CMOS Theory and Applications...

28 May 2024 9,284 0 View

Is there a Raising awareness of indigenous medicine among metropolitans?

The growing interest in holistic health practices is leading to increased awareness of indigenous medicine among metropolitan populations. While challenges exist in terms of access and cultural...

09 May 2024 7,041 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View