I would like to know about the cytometric analysis. Can we use this analysis as additional in confirming the cytotoxicity of a plant extract?
Absolutely yes. After exposure of the plant extract to cell line or PBMC, you can use flow cytometry for measure of cell death or viability.
In adition to the response of Ishak, you can also verify if the cell death was by apoptosis or by necrosis.
I agree with Adriano. You can use PI and annexin V for discrimination of apoptosis and necrosis by flow cytometry
thank u Ishak for your kind response.
You can think of analyzing by HPTLC protocols..
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