Can someone suggest how to link thioglycolic acid to the N-terminal of peptides that already cleaved from the resin?

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Afang Zhang

You could use active ester methodology, but need to protect the thio-group first.

Enrico Perspicace

Dear Sahar Ahmed,

Buy methyl (or ethyl) thioglycolate (it's not expensive) and use standard conditions EDCI/DMAP. It should work. Then, perform the cleavage of the ester with LiOH in a mixture of THF/H2O.

Good luck!

Regards