How to use protein A G beads to purify antibody?

Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...

22 February 2021 4,318 2 View

Amorphous silica XRD peak at around 10 degree?

21 February 2021 3,417 5 View

How could I get the P-value from the mean, SEM, and n of different groups in the t-test?

How could I get the P-value from the mean, SEM, and n of different groups in the t-test without the original data? For detail, I would like to know the P-value between BMP-2: 50ng/ml group and the...

06 February 2021 6,539 4 View

Why are my western blot bands so distorted?

Hi guys I'm out of ideas here please help! I've never had consistent problem like this doing westerns before and I can't figure it out. The condition used: - First 4 lanes have 50ug protein in...

04 February 2021 2,297 3 View

What mechanism is involved when chitosan is coated on magnetic nanoparticles?

Hello! I am currently working on coating of chitosan on magnetic Fe3O4 nanoparticles. I am wondering how this coating process happened? i.e. through what mechanism that chitosan can be coated on...

13 January 2021 5,657 5 View

The impact of different poisson's ratios when analysising field vibration?

The Poisson's ratio of soil is quite difficult for us geo-engineers to obtain. I came across a problem when I tried to determine the parameters in a FEM model. For the saturated clay in...

02 January 2021 8,971 3 View

What are the possible drawbacks of using miRNA sponge to enhance the specificity of gene expression?

I am searching for miRNA repeats put between WPRE and 3'LTR of my lentivirus, in order to enhance the specificity of transgene expression in human neuron cells. But if clinically translation of...

26 December 2020 9,921 2 View

Is it possible to deposition a large area dielectric layer(mm scale) on the wet transferred graphene?

I want to deposition a dielectric layer on top of the CVD wet transferred graphene. The purpose of the dielectric layer is to uniformly cover the graphene and enable the top-gate modification of...

14 December 2020 7,693 2 View

Is the fluorescent protein must be totally photobleached in FRAP assay?

Hi I have tried to measure the mobility of a protein of interest by the FRAP assay. The protein was fused with GFP. I overexpressed the fusion protein in Hela cells. However, when the area of the...

14 December 2020 6,567 1 View

Can Apparent Activation Energy determine Conversion Rate in a catalysis reaction?

Recently, I got a concept that is contrary to my understanding from two papers. It can be described as follows, in brief: There are two catalysts for the same reaction, on the catalyst with higher...

09 December 2020 2,406 28 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How much cell pressure and back pressure should I apply to start for triaxial test for different types of soil?

We have recently purchased a triaxial test instrument (MATEST) for testing different types of soil. After installing software, we are facing a issue of saturation where the samples does not...

23 February 2021 7,194 4 View

Is it possible to prepare clay minerals by mixing pure oxides of required elements? What would be theoretical difficulties with it?

Practically this is not at all necessary. But theoretically, would hydrous/hydrothermal synthesis be enough (like mix 1 mole alumina gel and 4 mole silica gel and gently heat) or would it require...

26 January 2021 6,403 3 View

Do you have information about synthetic diamonds and their environmental impact?

I am doing a deep research on the comparison of the process of extraction of natural diamonds and synthetic diamonds, and their environmental impact I would appreciate you could share your...

19 January 2021 1,359 3 View

Johnson et al., 2006 Geology of South Africa?

I am wondering if there is a digital (PDF) version of this book available or if anyone is willing to share this work. It is no longer available from GSSA (Geo Soc. S. Afr) nor does my library hold...

13 January 2021 5,571 2 View

Is there any chemical environment or parameters to define or explain the sediment and soil profiles, are they separated by chemical parameters?

Sediment is relatively younger than the soil in the depositional environment as the sediments are consequence of the accretion of particles transported either by waters or by winds, whereas, soil...

12 December 2020 4,822 18 View

How to model a graphite-bearing rock using Perplex (pseudosections)?

Dear all, I am struggling to model a rock which contains the assemblage K-white mica + Na-white mica + chlorite + quartz + graphite + rutile. I have followed step by step the tutorial available on...

22 November 2020 2,387 2 View

What is New Next Generation Attenuation Relationships (NNGAR) and how are they different from other attenuation relationships?

Dear researchers The provisions of ASCE 7-10 states that New Next Generation Attenuation Relationships (NNGAR) has been used in the process of Probabilistic Seismic Hazard Analysis (PSHA) to...

15 November 2020 1,132 4 View

Survey on geology ?

Kindly request you to spare couple of minutes in filling up the form/survey. It is for a study/research purpose. Jishnu B...

22 September 2020 7,358 7 View

Any Book or Article on Pleistocene Planktonic Foraminifera and Calcareous Nannoplankton?

Dear all, I am working on the biostratigraphy of Pleistocene sediments from the high latitude region in south-east Indian ocean sediments. Kindly suggest me any book or articles in which the...

09 September 2020 1,544 9 View