Should GFP sequence be cut together with the IRES sequence?

Hello. I have a viral plasmid which contains a bicistronic region: CMVprmtr-GFP-IRES-PuroR. I would like to cut out the GFP sequence to get rid of the green fluorescence. In this case, would it be...

02 February 2021 1,644 7 View

Examples of SWAT used in water management in operational practice?

Do you know any examples when the hydrological model made using SWAT became some software product used in operational practice for water management (cases of the water reservoirs management are...

04 June 2020 4,191 1 View

What are these cells with highly condensed basophilic nuclei and a light cytoplasm between proximal tubule epithelial cells in the zebrafish kidneys?

Please help determine what cells are in the proximal tubules of zebrafish. They have highly condensed nuclei, but I suspect that this is not karyopiches of apoptotic cells, but mitosis of normal...

03 June 2020 4,093 5 View

Can anyone suggest me any soft for chromatograms viewing and processing (UV MS PDA) which I could use at home?

Can anyone suggest me any soft for chromatograms viewing and processing (UV MS PDA) which I could use at home (freeware)? Chromatograms were made by Waters UPLC I-Class PDA-MS. At the workplace,...

14 May 2020 5,383 3 View

No title

It is quite difficult to find these enzyme preparations at that moment. Can anyone tell me who I can contact on this issue with?

29 February 2020 2,130 3 View

How to dilute fish blood plasma?

We are encountered with a phenomenon when T3 FREE was estimated in fish plasma by ELISA (DRG tests): some samples are shown no significant differences in total and diluted plasma (in PBS pH7.4,...

29 September 2019 8,819 3 View

Does anybody know a database on fluorescent properties of chemical compounds?

Does anybody know a database on fluorescent properties of chemical compounds? There are many papers describing flurescent properties of various compounds. Is there any database on fluorescent...

06 August 2019 9,239 3 View

How shoud one switch off a rotary vane pump?

How shoud I switch off a rotary vane pump? Should I initially turn off the pump and then open the shutter to normalize the pressure? Or the order of the actions should be vice versa?

06 August 2019 9,982 3 View

Can anyone verify whether this is an artifact or a point mutation in these Sanger sequencing results?

Hello! I have really strange results in my sequencing chromatograms. The goal was to analyze a point mutation which is heterozygous (DNMT3A gene, in position R882). So I performed a Sanger...

17 May 2019 7,386 3 View

Good day! Can someone help me? How can the mixing speed be taken into account in the mathematical model of diffusion in a liquid medium?

Good day! Can someone help me? How can the mixing speed be taken into account in the mathematical model of diffusion in a liquid medium?

19 March 2019 7,162 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View