In my gc ms experiment I have got long chain monomers c15 to c19 while using linoleic acid as a carbon source in pseudomonas putida .Can p.putida capable synthesize long chain polymers?
Yes, definitely. It will store this as a reserve carbon source and use it when other readily metabolizable carbon source become scarce in the environment. It has to do so as it non-sporing and this is the only way it will survive.
The reply by Professor Noda is very fitting; Professor Alexander Steinbuechel is an authority on Biopolymers synthesized by microbes and the reference of that publication of 1995 is an excellent source of information on PHA. I have worked on a short publication on PHV some time back..
thanks a lot abdelkader, and according to me there is no point in late on sharing, the knowledge, infact, now I am getting more enthusiastic to discuss about the PHAs.Monomers it would b every nice , if we continue our discussion in the analysis part also i.e GC MS, NMR etc
it is a great honnor and privilege for me to take more knowledge from you and I am ready for any discussion within my modest background. Sincerly Regards
The answer is YES. P. putida can metabolize and degrade long chain hydrocarbons and fatty acids. During the beta-oxidation hydroxyl fatty acid are formed at various chain length and can be incorporated into a poly-hydroxy-alkanoate polymer. In most cases C8, C10 and C12 are the major constituents of such PHA, but it all depends on conditions especially on the affinity and selectivity of the synthase enzyme responsible for the polymerization.