In my gc ms experiment I have got long chain monomers c15 to c19 while using linoleic acid as a carbon source in pseudomonas putida .Can p.putida capable synthesize long chain polymers?
Dear Sir;
I hope the following attached files will answer to a great extent your question. Regards
Aromatics in petroleum include the monoaromatic
hydrocarbons such as benzene, toluene, and ethylbenzene,
and o, m, and p-xylene isomers (BTEX) and other alkylsubstituted
benzene compounds (Cn-benzenes), and polycyclic
aromatic hydrocarbons (PAHs) (which include oil-characteristic
alkylated C0- to C4-naphthalene, phenanthrene, dibenzothiophene,
fluorene, and chrysene homologous series and other
United States Environmental Protection Agency (US EPA) priority
PAHs).
There are different type of PHA molecule so i have send one paper which include different type of biopolymer
You may want to consult the following:
Engineering the Monomer Composition of Polyhydroxyalkanoates Synthesized in Saccharomyces cerevisiae
Bo Zhang,
Ross Carlson† and
Friedrich Srienc*
Mini-Review: Biosynthesis of
Poly(hydroxyalkanoates)
JINGNAN LU, RYAN C. TAPPEL, AND CHRISTOPHER T.
NOMURA
Department of Chemistry, State University of New York-College of
Environmental Science and Forestry, Syracuse, NY 13210, USA
The reply by Professor Noda is very fitting; Professor Alexander Steinbuechel is an authority on Biopolymers synthesized by microbes and the reference of that publication of 1995 is an excellent source of information on PHA. I have worked on a short publication on PHV some time back..
Dear Dr. Ahil;
I hope it is not too late. The following attached book is an extremely important one in the area you are working in. Regards
thanks a lot abdelkader, and according to me there is no point in late on sharing, the knowledge, infact, now I am getting more enthusiastic to discuss about the PHAs.Monomers it would b every nice , if we continue our discussion in the analysis part also i.e GC MS, NMR etc
Dear Ahil;
it is a great honnor and privilege for me to take more knowledge from you and I am ready for any discussion within my modest background. Sincerly Regards
Ahil,
The answer is YES. P. putida can metabolize and degrade long chain hydrocarbons and fatty acids. During the beta-oxidation hydroxyl fatty acid are formed at various chain length and can be incorporated into a poly-hydroxy-alkanoate polymer. In most cases C8, C10 and C12 are the major constituents of such PHA, but it all depends on conditions especially on the affinity and selectivity of the synthase enzyme responsible for the polymerization.
- Badges
- Science topic
- Similar topics
- Physical Sciences
- Materials Science
- Materials
- Polymers