What is the best method for lipids extraction of leaves?

Hello! I need to extract lipids from three plants. What is the best method { If you can help me with a protocol, thank you regards

27 April 2018 5,853 4 View

How are emission factors (CO2e)for solid waste components calculated? I believe LCA is involved but Im not sure how ?

07 August 2017 6,340 4 View

Can someone provide instructions on how to correct LA-ICP-MS data?

I have averaged my elemental intensities, I have my std values , created correction factor... Thank you!

07 August 2017 5,510 4 View

What are the main challenges of direct 3D printing with precious metals?

I am doing my dissertation on mass customisation and engineering of jewellery products. One of my aims is to find out why direct 3d printing in particular in the jewellery industry has not been as...

06 August 2017 6,323 9 View

What is the acceptable number or outliers in a research?

18 December 2016 8,596 17 View

How do I aggregate PLA-PEG nanoparticles?

Hello everyone!! Currently, I am working on the fabrication of PLA nanoparticles with PEG adsorbed on their surface. I am based on the article "The internalization of fluorescence-labeled PLA...

18 December 2015 2,751 3 View

Can someone identify the eggs found in faeces of the river Otter, Lontra longicaudis?

Our Group (Laura Jaramillo and me) have two other parasite eggs found in faeces of the river Otter, that are different of the previously discussed

17 February 2015 4,916 5 View

Can we really measure test-retest reliability in memory tests?

It seems obvious that there is a big difference between being repeatedly exposed to a reaction time task, in which minimal or any learning at all is expected, and to be evaluated several times...

09 October 2014 8,615 12 View

What is the most difficult factor for elaborate Biodiesel?

The main factors for produce bio fuels

23 April 2014 3,230 4 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

How does elongation affect the resistance of an electrode?

What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.

22 February 2021 1,515 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View