Treatment of a large cross sectional unit in a panel setting?

I have a data set of 5 countries for 30 years and i want to analyse the relationship between y and x in panel setting. However, the countries are not similar in nature moreover. one country is...

30 January 2021 6,564 4 View

What is the solubility of Magnesium trifluoromethanesulfonate in methanol?

I am trying to dissolve Magnesium triflate in methanol. I took 2.6 g of it in about 20 mL methanol. After thorough stirring, I can see suspended particles! Can anybody throw some light on its...

12 January 2021 6,481 2 View

Could you explain the following situations, please?

I am trying to simulate a flexible antenna on ANSYS HFSS. Here, I have used "PDMS" as a substrate and "pec" as a probe (inner conductor) of a coaxial feeding. But during simulation, it is showing...

24 November 2020 800 5 View

Can I get an explanation for the following reason, please?

My plan is to fabricate a conformal microstrip patch antenna. I am going through the simulation works first. I already simulated the planar antenna. Now I am trying to simulate it with different...

19 November 2020 2,961 2 View

What are the community-based primary prevention of NCD in LMIC country?

Please suggest types of literature to look for? Recommend journals which focuses on these issues.

12 October 2020 2,878 4 View

How write a concept note on Evaluation of community-based primary prevention models for NCDs?

1. What type of literature should I read for writing a successful concept note? 2. Is there any literature which will help me decide a research question/objective for designing a study will be...

12 October 2020 3,363 5 View

Which software might be helpful to design and simulate the 3D shape of the planar/nonplanar microstrip/any antenna?

I am experienced with the antenna simulation using ANSYS HFSS and CST. But now I am working in an additive microsystems lab and want to move to 3D shape antenna simulation and printing.

03 August 2020 2,184 3 View

If I want to print the microstrip antenna using additive manufacturing techniques, do I need to design a 3D shape on CAD that I have created on HFSS?

I have designed and simulated a frequency reconfigurable on ANSYS HFSS, and now I want to print it using an inkjet-printing technology.

03 August 2020 7,439 4 View

What will be the best methodology to quantify Demographic Dividend and Skill labour of any country?

Most of the researchers used panel data to show the impacts of demographic dividend. But for individual country where divisional or district data over the year are not available, what methodology...

29 July 2020 6,733 1 View

Discrete Element Method Simulation Example Using Ls-Dyna?

I am interested to simulate the fracture process of glass using DEM in Ls-Dyna and need some example or bench mark case for learning.

21 July 2020 4,156 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Agarose gel purification pre MinION sequencing?

Dear all, we performed an agarose gel purification with MinElute kit (QIAGEN) to extract bands between 3500 bp and 6000 bp for further sequencing with MinION. After the purification process,...

16 February 2021 5,178 1 View