This is so confusing especially that standard PCR is like RT-PCR in recent studies.
Please Compile this answer in a table format.
I made my own but it still not impressive.
visit this link below:
https://www.enzolifesciences.com/science-center/technotes/2017/march/what-are-the-differences-between-pcr-rt-pcr-qpcr-and-rt-qpcr?/
Hello Morongwa EUNICE Kgomo
For the better understanding of the three terms I have tabulated in the following manner -
RT - PCR
RT -qPCR
Q-PCR
1) Reverse transcription polymerase chain reaction is a laboratory technique combining reverse transcription of RNA into DNA and amplification of specific DNA targets using polymerase chain reaction.
2) It is primarily used to measure the amount of a specific RNA.
1) Commonly, in RT-qPCR, RNA transcripts are quantified by reverse transcribing them into cDNA first, as described above and then qPCR is subsequently carried out.
2) As in standard PCR, DNA is amplified by 3 repeating steps: denaturation, annealing and elongation.
1) Q-PCR or Quantitative PCR is a method by which the amount of the PCR product can be determined, in real-time, and is very useful for investigating gene expression
Hope, I am clear with my explanations
Regards
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