Urine usually contain -OH products, as glucuronides. 30 or 15 mL urine + 1M sodium acetate buffer pH 5.5, + 0.1 mL glucuronidase/arylsulfatase, mix, left over water bath at 42 C for 24h, stop the reaction using 500 uL CHCl3, mix. Extract 15 of this solution using extrelut column (sea my publication; Famprofazone, to know exact procedure of enzymatic hydrolysis) and extraction.

Analyze using LC-IT-MS/MS. try to extract EIC; extract ion chromatogram of expected -OH, acetyl, derivatives.

Is there any software to predict these metabolites