Multiplex qPCR annealing and extension?

Dear All, I have set up a 5-plex qPCR. The multiplex works fine but the PCR efficiency doesn't look that great. I am throwing in 250ng human genomic DNA and I get Ct in range of 30-35 for all my...

13 February 2020 7,095 3 View

Is it feasible to use RNN model for improving the visual quality of the image(image enhancement)?? I am unable to find any paper related to this?

I am working on image processing especially in the area of image enhancement. But I am unable to find any paper related to RNN model for improving the quality of the image.

11 February 2020 140 2 View

RNA extraction from MTB?

Dear All, I am looking for a kit to extract total RNA from MTB which I will later use for RT-qPCR. My initial sample would be very low and cannot afford to loose RNA during the extraction. Also,...

09 July 2019 1,278 5 View

How to store root tissue for a week which is already treated with DAF-FM DA for NO fluorescence estimation as microscope is not available?

The root tissue is already incubated in the DAF-FM DA and washed with HEPES-KOH buffer. But due to non-availability of confocal microscope on the same day, is it possible to store the tissue in...

26 July 2018 847 2 View

What tests can be used for descriptive research?

Hi, will appreciate expert opinion on the below: 1. Can 'impacting' or 'relationship' hypothesis be formulated for descriptive research? 2. Can correlation, regression and chi-square tests be...

27 March 2018 4,858 7 View

Continous column adsorption capacity

If i am working on a column filled with adsorbent, in my batch study max adsorption capacity was achieved in 1 hr. In continous column how can i achieve that max adsorption capacity that was...

26 June 2017 4,191 7 View

In my model of matlab for fixed bed adsorption column, i have obtained very large time in X-axis of breakthrough curve?

In my model of matlab for fixed bed adsorption column, i have obtained very large time in X-axis of breakthrough curve..i.e nearly 5*10^(5) second. Is it possible? why it is higher. please suggest?

03 May 2017 4,295 3 View

What solvent system i should use for running a TLC for water soluble compound ?

i have a bacterial crude extract soluble only in water .........what solvent system i should use to run TLC or column

10 February 2017 499 4 View

Positive delta G?

07 January 2017 5,042 3 View

Can pikovskaya isolated bacterial colonies be transferred on nutrient agar plate ?

I have isolated bacterial colonies on pikovskaya agar plate but when transferring them to nutrient agar plate as well as nutrient broth for purification they were not growing

26 April 2016 9,360 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Statistics: include groups of respondents made by one person for analysis?

Good morning, I could not find literature about this so I am asking. I am organizing data for a questionnaire (N=100) that foresees some personal questions like level of education. For the aim...

25 February 2021 2,090 4 View

Sample size determination for non-parametric tests?

How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...

24 February 2021 166 6 View

Statistical Tests for Quantitative Study?

Hello Everyone, I have one population, sample size 200. and needed to find the correlation between Variable A and B. Also, which T-test should I choose for hypothesis testing Please advise...

21 February 2021 7,591 4 View

How to model rupture in geogrid in abaqus?

Hi researchers I wanna model tensile test on geogrid in abaqus, so I added displacement to one side of geogrid and the other side of geogrid is constrained completely, everything seems normal but...

19 February 2021 2,195 2 View

How to compare means of continuous data with zero-inflation and skew?

I have two groups (unpaired) of plant biomass measurements and want to know whether there is a test that can be used to determine statistical significance where assumptions are not violated. The...

18 February 2021 1,258 4 View

How to report Fishers Exact test from SPSS in a report properly?

I am wondering how do I write the data I have collected from my fishers exact test into words so that it backs up my work? the exact sig.(2-sided) was 0.033, meaning it is significant because its...

17 February 2021 3,441 7 View

ANOVA or Paired samples t-test?

Hi, I'm a masters student struggling with the design of my cognitive psychology project. We wish to run an experiment where we examine false memory recall using a list of schema related nouns in...

13 February 2021 6,457 3 View

How do you calculate a discrimination index for polytomous data?

Hello, I'm currently running a PCA on a polytomous data set (5 point Likert). I want to create a discrimination index to narrow down my items before running the PCA. After looking around, I'm...

13 February 2021 3,517 2 View