I have adult zebrafish that were snap-frozen with liquid nitrogen.
Does your question refer to paraffin-processing (FFPE) of unfixed frozen tissue?
you can make this with cryostat.
chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/https://www.ehs.harvard.edu/sites/default/files/cryostat_safety_SOP.pdf
If you snap freeze unfixed tissue, you should use a cryostat and section it. Both immunostaining and histological staining can be performed. Taking snap frozen tissue and then processing it for histology will probably lead to morphological changes mainly because of extreme temperature changes going from -70 to +4 during fixation. Also, alcohol dehydration would damage non-fixed membranes, probably destroying tissue integrity.
We routinely do ISH and IHC on our snap frozen brain tissue. We embed in OCT and section in a cryostat. We usually do RNAscope on these just fine, but I recently did both H&E and IHC on snap frozen sections with relative success. You will be dealing with 'sub-optimal' tissue, but it is definitely possible. I use a combination of many different IHC protocols (including the one specific to my antibody).
Frozen Tissue Preparation & IHC Cryosection Staining Protocol: R&D Systems
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