Can recommend reliable melting point apparatus model?

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Is it okay to test toluene in HPLC at 190 nm?

Hi everyone..I tried to analyse toluene in HPLC for low level at less than 1% by HPLC at 190 nm.I prepared toluene standard by weighing 0.02 g in 25 mL diluted with acetonitrile followed by...

07 February 2024 5,636 6 View

How to optimise a method when using column with different pore size?

We were trying to develop an HPLC method as an alternative to the titration method for active quantification for one of our products that contains two actives. We managed to develop the method...

01 June 2023 1,166 4 View

Can I shorten an HPLC run time based on the retention time of the target compound without waiting for others to elute?

Say I am analysing a sample containing analyte A and B with the retention time of each at 5 minutes and 11 minutes. If my target compound is analyte A, can I shorten the run time to 7 minutes or...

18 October 2022 831 5 View

How to determine LOQ and LOD of titration?

Dear all, I used this link as guidance for titration (https://pdf4pro.com/view/validation-of-titration-methods-bikeitech-com-5a2e5f.html). According to this link, the series of solution with...

07 October 2020 3,137 7 View

Is using hydrogen or nitrogen as alternative to helium giving a reliable results?

I am in the midst of surveying GC models to buy.One of the model offers technology that uses hydrogen or nitrogen instead of helium. This obviously will be cost saving but how about the...

27 June 2020 1,452 1 View

Which brand of GC/MS is ideal?

We are considering to buy GC/MS. Can experts out there share your review with your current GC model?

25 June 2020 6,341 21 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

What is the prepared reference material that can be used in inductively coupled plasma optical emission spectrometry (ICP-OES) to measure REE and U ?

What is the prepared reference material that can be used in the ICPE-9820 Shimadzu Japan instrument, which employs inductively coupled plasma optical emission spectrometry (ICP-OES) to measure...

06 August 2024 1,896 1 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

How can I add own Henry coefficients in Aspen Plus?

Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?

05 August 2024 2,333 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

Melt curve problem in qPCR?

Do these peaks in the curve indicate primer dimer or non-specific products?

01 August 2024 7,466 0 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.

31 July 2024 9,140 2 View