I am studying the controlled release of eucalyptus oil from microbeads. How can I quantify the amount of the oil release into solutions using the spectrophometer?
Hi Jubril,
As you know essential oils are complex mixtures of several components what makes their characterization difficult. The UV absorption spectra of oil samples in ethanol may show the presence of tannins, the peak often observed at 200-280 nm is typical for condensed benzene ring system. In addition, you can observed another band in the range of 200–210 nm for the electronic transition n→π* of ester carbonyl group present in some components.
The problem in quantifying the volatile being release using UV-VIs and making a calibration curve is that you will see bands resulting from several compounds and not by a single "model molecule". Therofere I think you should use HPLC to quantify one or more species being released for example in ethanol.
I will find many papers published on this.
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