I am busy with humanized mice for the study of PK/PD of antimalaria drugs. The work is iinvolving, engraftment of mice with human red blood cells (hRBCS), thereafter induce blood stage infection with plasmodium falciparum parasitized RBCs at a specific concentration. Knowing the concentration is important to monitor (by flow/elecron microscope) if the parasites growth reproductively in their artificial host. My question is to know, how to accurately work out this concentration using trucount tubes?
I don't have personnel experience with trucount, but my understanding is that they are meant for lymphocyte, not RBCs. I assume, you'd need to enumerate infected red blood cells. That can be done using Giemsa-stained thin blood-smears and a hemocytometer. Otherwise, there are different flow cytometry protocols to monitor parasitemia/iRBC concentration.
Thanks for your reaction Whatsala. Based on literature this technic also applies for the enumeration of parasitized RBCs.
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