NUMT is responsible for migration of Mt components into the nucleus of treated living cells...
We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.
08 August 2024 3,161 0 View
a) Design a scalable and robust network architecture that can handle the increasing data traffic and support various communication technologies. b) Recommend suitable transmission technologies,...
10 June 2024 2,187 1 View
need details
20 April 2024 4,660 0 View
I need more articles on kaolinite clay and the same clay treated by number of geotechnical methods
14 February 2024 607 2 View
Pressure data has been recorded at each crank angle in a diesel engine, and I couldn't able to understand how to calculate the temperature from that data. is it possible or any other way to...
24 January 2024 2,926 3 View
Dear All, As I mentioned in the question itself these two beverages are saught after worldwide as a refreshing drink. Though, I like both tea and coffee the personal preference on day to day...
02 November 2023 2,011 8 View
I am new to WIEN and have been reading and looking at youtube installation instructions. However, I have not been able to make a clean installation. Errors start with SCF calculations where dstart...
02 September 2023 9,675 0 View
??
22 August 2023 4,341 4 View
purpose of reference electrode and counter electrode in supercapacitor?
22 August 2023 2,710 3 View
I have to detect glutamine from a biological sample using HPLC/LC-MS. However, glutamine is very polar and doesn't bind to the column nicely. I used Fmoc to derivatize glutamine so that it can...
10 August 2023 5,839 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
I need to install this program
25 July 2024 4,756 0 View
In general, when performing a Tafel analysis for corrosion resistance measurements, a line voltage scan is performed from say -250 to +250 mV vs. OCV at low scan speed around 0.125 mV/s. However,...
25 July 2024 7,982 3 View
I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...
25 July 2024 6,517 3 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View