Selecting particular columns from large datasets (csv) using R Studio?

So I have large number of csv files (100+) in a directory with same structure and column names. The data is in time series and there are approx 30 columns/file. However i only want to aggregate...

08 February 2021 245 5 View

How can solve the second order linear homogeneous partial differential equation with variable coefficients?

can someone help me with reduced the partial differential equation du/dt = - f(x) d^2 u/ dx^2 - g(x) du/dx --- (1) to become: du/dt = - p(x) d^2 u/ dx^2...

21 January 2021 3,296 1 View

IHC-paraffin experiment: how do you approach shopping for an antibody?

Hello everyone! I am shopping for an arginase 1 antibody to use for IHC-paraffin experiments with rat tissue. So far what I have understood is that if an antibody says it is reactive with rat,...

16 January 2021 794 3 View

How to explain insignificant independent variable which is significant with interacting (Moderation) variable?

10 December 2020 3,037 1 View

What could be the impediments to the initiatives adopted by the bank towards FI in the Indian scenario?

this question from case Union Bank of India: Initiatives towards IT-enabled Financial Inclusion

09 December 2020 7,721 3 View

Can we use the general hotplate as a source of heating in the Soxhelt method or not ? what is optimal temperature when using n-hexane as a solvent?

there is a problem with the heating part in soxhelt apparatus, so my question can we use the standard hot plate as a source of heating?

05 December 2020 9,322 2 View

Carbon Emission calculation factors ?

I am thinking about writing a paper on carbon emissions based on electricity consumption data from some buildings. From a research perspective, which factors should i specially focus while...

09 October 2020 4,142 3 View

How to calculate the quantity of enzyme needed to hydrolyze protein?

I have alcalase enzyme it's activity 10000U/g and I want to use it in hydrolysis whey protein isolate 90% protein. A suspension of 5% (w/v)WPI prepared so I am asking about the quantity of enzyme...

05 October 2020 9,825 3 View

Will the publishing of conference papers be counted?

During promotion, papers published as conference proceedings are not considered; on the other hand, it is counted as publications in a few places but with less credits. kindly request your...

23 September 2020 3,254 38 View

Which configuration should be more preferred AC or DC coupled for PV-battery storage systems?

The ideal assumption is : uniform sized PV and battery storage system, and similar load for both configurations.

07 September 2020 7,193 30 View

RWP1 growing in RPMI medium?

Hello everyone, Has anyone try to culture the normal prostate RWPE-1 cell line in RPMI medium supplemented with foetal bovine serum? There's some articles available but neither describe details...

08 February 2021 6,455 1 View

Scanning Electron Microscopy for Extracellular Vesicles?

Can anyone provide or recommend a detailed protocol for preparing extracellular vesicles for SEM? Most protocols that I have found that list the necessary reagents and individual steps are for...

02 February 2021 4,475 3 View

Is there any possibility to buy specific drug resistant human and mouse tissue array from company or other research organisations?

I need some specific cancer tissue samples' slides for IHC which is resistant and sensitive to specific cancer drugs, especially for prostate, lung, and breast cancer. Is it possible to buy or...

27 November 2020 731 2 View

Which ABs concentration for EVs immuno-staining?

Hi all, I have to do a phenotyping study on cell culture derived EVs, in particular I should stain them using primary labelled ABs anti-EVs markers before proceeding to analyze them through...

25 November 2020 4,601 1 View

What are the morphological changes of PC3 cells during autophagy and/or apoptosis?

I was performing wound healing assay seeding 300,000cells/well in 6 well plates with 2mL RPMI medium. At 0hr after intervention, the cells looked like the first picture. At 24hr after...

18 November 2020 9,671 2 View

How to extract extracellular vesicles from cell lines?

Recently, there was a problem with my qpcr results. I want to try more methods of extracting EV from the cell medium. At present, I am using Invitrogen's kit(Cat4478359) to extract exosomes. Can...

28 October 2020 2,961 3 View

Can I get exosomes from cryopreserved whole blood supplemented with concentration of DMSO (10%)?

I read many papers about exosome isolation from fresh blood or frozen plasma, but not for frozen whole blood. I am wondering whether can I get exosomes from cryopreserved whole blood? One of my...

26 October 2020 5,166 3 View

What statistical test should I use?

Hi, I am trying to choose the appropriate statistical test to evaluate cells (drug vs control) over 4 different time points (inc. 0hr). Specifically I was testing prostate cells treated with a...

12 October 2020 2,870 19 View

Should antiandrogens and / or TMPRSS2 protease inhibitors be given in adult patients without prostate cancer and also in women? Only in men? Never ?

As we know, not long ago the WHO named Wuhann Pneumonia (SARS-COV 2): Covid-19 (from Coronavirus Disease 2019), which has become a serious pandemic and health emergency with multiple pathological...

21 September 2020 4,618 13 View

Which wedium do we use for prostate cancer LNCaP cell line?

Dear Scientists, I am a Master student and I am doing an anti-cancer effect on LNCaP cell line. I have not had any experience with this cell line before. I found on ATCC and some papers, they...

14 September 2020 3,375 6 View