Hello,

I'm growing different accessions of Arabidopsis thaliana and so far we have been harvesting them by pouring the media over a simple sieve, then putting them on paper towels for a minute to try to remove excess media. However, too much still remains and the samples become total rocks in the freezer and it makes metabolite (MeOH) extraction really difficult.

Is it okay to use soft vacuum filtration for a few seconds to harvest the seedlings? Which type of membranes can I use with 0.5 MS (pH 5.7) media? Our department generally stocks PES.

Or maybe I should do the extractions right away?

Thanks,

Casey

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