If there is any suggestion that the primates tend to avoid roads or their vicinity (even at night when traffic might be lower), then I wouldn't adopt this strategy as it will significantly bias your outcome. For a more representative sample, it would be better to follow a transect through the actual range of the primates under study (or through less disturbed habitat if you have no idea what the ranges of the primates might be). However, if time and resources allow, it could be informative to include at least one road survey and, in that way, you can reveal if your primates avoid roadside habitat.
hi, the methodology of your desing is crucial. For an index in a panel study this might work. If you want to calculate the abundance out of these samples, your transects have to be real random samples. And if you want to use an mathematical estimator for the abundance like in 'distance', responsive movement should not occur