I will be measuring SOD activity in plant samples. I will use the Becana et al. (1986) method to determine the SOD. We currently don't have NBT in our lab but we do have INT. Could I replace one for the other? The NBT is quite pricey and I don't know if we will need to use it again....
I think you should use NBT, the structure of NBT and INT are quite different, and there is the distinction between the reaction mechanism. The NBT is the traditional classic spectrophotometric method . The result from NBT was the most convincing possibility. That is just my private opinions and hope to help you .
Raja Awan The alkaloids played a determinant role in datura alba leaf ,for example, it suppressed the activity of AchE, and attack the nematode neural system. However, the extraction process is complex and the yield is low , not available in large quantities , artificial synthesis can obtain a large number of products, and high purity .
Datura act as a anti-feedant and ant-repellent to insects because it have more alkaloids.
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