I found an un-opened bottle of expired (2015) KO-SR (serum replacement) for iPSCs at -20C in my lab. The catalog number is 10828028 from GIBCO. We are facing some funding issues so it is hard to convince professor for buying something. In this situation, can I use that serum for my work? Is there really something in medium which gets expired even by keeping it only at -20C?

It was ordered by my seniors long time ago and never thawed.

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