I want to measure hydroxy radical (OH) using ESR spectroscopy. From the literature, they say DMPO-OH adduct detection will get interference from transition metal? Since my probe contains iron, I wonder can I use this DMPO for detecting OH radical or I have to go for BMPO?
Axel Klein
I would say: YES.
There is good report by Schlick et al. using DMPO for trapping OH. generated from H2O2. However, since there is never a guarantee that the method reported there is also suitable for your purposes, you should also think about other traps such as PBN or BMPO.
The problem is the high reactivity of the OH. radical.
Good luck
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