How does the N value be counted for gut content analysis?

I read some articles on mollusc and fish diet analysis. The formula for percentage frequency of occurrence stated above: %F0 = Nei/Ne x 100 and percentage numerical abundance : %NI : Ni/N x...

15 October 2020 4,568 3 View

Why is it necessary to vortex and incubate in dark the sample and DPPH solution and other DPPH questions?

Hi all, In the determination of Antioxidant capacity of fruit samples, I used DPPH assay and have a few questions regarding the DPPH assay 1) why is it necessary to vortex and incubate in dark...

30 August 2020 246 3 View

How to renew the Scopus index status of a journal?

Dear Researchers, Our journal (ISSN no. 2070-4658) was part of the Scopus index till 2017. Now, it is not in the Scopus index as the last two years no issue has been published. However, the...

24 August 2020 7,203 3 View

What strategies would be effective to handle mixed-ability students in learning English as a foreign language?

08 June 2020 1,850 32 View

Hello,i extract my plants using rotary evaporator and i got crude extract.How should dilute it?i have no idea on how to dilute it?Can someone helpme?

dilute it in ethanol or dmso? which solution is better as i used 95% of ethanol as my extraction solvent?

23 March 2020 5,971 6 View

Can I set "20 uL reaction volume" in PCR cycler machine, while my actual sample is only 10 or 15 uL?

This is a stupid but quite technical question. We have a PCR machine that has 20 uL minimum reaction, so it can't be set into 15 or 10 uL in the machine. Is this going to affect the result...

22 March 2020 3,497 4 View

Is there any SOP to measure pharmaceutical clean room and if none can we develop our own method to measure the room size?

06 March 2020 866 1 View

How to test degree of polymerisation of aged kraft paper impregnated in oil?

kraft paper was impregnated in mineral oil at different temperatures and duration. my project is to get the DP value at different duration. in order to be measured by viscometer, the sample must...

20 January 2020 2,234 3 View

What is the equation to explain how one possible organic side product is formed in the synthesis of 1-bromobutane?

And show the chemical properties window of this side product. Explain how this product can be separated from the desired product. I have already identified that the possible organic side products...

29 December 2019 6,170 4 View

Validation for Intracerebroventricular Microinjection of Streptozotocin in Rats?

Hi, I'm planning to induce sporadic Alzheimer's disease (AD) in male Sprague Dawley rats (3 months old, 270-350g) through bilateral intracerebroventricular microinjection of 3 mg/kg streptozotocin...

25 November 2019 9,723 3 View

Buillding 3D Model Fussion Protein with Modeller?

Hello May i ask you about using Modeller for making 3D model fussion protein. I want to build a fusion/chimera protein structure which is including 2 different protein and then evaluate quality...

09 February 2021 9,369 2 View

Can we incorporate unnatural amino acids into protein using software for graphical image purpose?

I am looking to incorporate a unnatural amino acids which is not available in the swisssidechain database into a protein crystal structure of pdb? I am using chimera 1.14 vis. tool?

26 January 2021 4,064 3 View

Best tool to build missing residues, minimize and validate a ligand model?

I am working on a ligand that is co-crystalized to parotein, but the ligand is missing some residues that makes it appear as if it was separated into two ligands! what I need is to connect them...

23 January 2021 3,975 7 View

How to extract 300 dpi images from Chimera X?

Recently I have started using ChimeraX, version 1.1. Unfortunately, I could not find any options to export images at a resolution greater than 96 dpi. I have tried the steps written here...

29 November 2020 4,982 4 View

How to use a .pdb file as structure template to modeling on Chimera 1.14 ?

Hi, I need to use an own file as a template to modeling on Chimera (using Modeller). When I try to blast my sequence only find the option to query structures available on PDB Web Site. Does...

26 October 2020 2,436 3 View

How can I convert a CHIMERA session file to PDB file format?

Hello everybody! Recently, I have joined two peptide fragments by incorporating a peptide bond between their N and C terminal using the standalone USCF CHIMERA software. The file is now saved as a...

23 October 2020 1,652 3 View

Energy minimization of a protein structure with acetylated lysine residues?

I performed in-silico acetylation using PyTM plugin but there are certain clashes that I want to resolve. I couldn't perform energy minimization using gromacs, chimera as they do not have default...

26 September 2020 3,907 6 View

Steps to improve cryoEM helical reconstruction resolution using relion?

Hello all, I am trying to do helical reconstruction for my fibrils. I am at 3D classification steps where I can get the resolution (given by relion) at 3.2 A. The actual resolution is worse I...

28 July 2020 6,266 1 View

What solutions do you suggest for molecular field visualization/analysis?

Hello, I wonder what options exist these days for visualizing "molecular field" type data. That is, a 3D scalar field represented by translucent volume rendering or isosurfaces, and overlaid on a...

12 June 2020 861 2 View

Why the ZDOCK output file (.pdb) is not compatible for Chimera?

Why the final docked complex file output from ZDOCK is not compatible for Chimera?

24 April 2020 3,347 3 View