Can I measue soil cellulase activity with dns ?

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Osama Rahil Popular answer

Please see the following article

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6032522/

Best Regards Baraa Rehamnia

Osama Rahil

Archana Mishra

Nope. DNS is a method for the estimation of Total reducing sugars, not for enzymatic activity measurement.

Baraa Rehamnia

@Archana Mishra By detecting the produced reducing sugars from the degradation of cellulose. Maybe we can mesure the enzymatic activity as follow

@Baraa Rehamnia yes you can measure the enzyme activity by correlating it with total reducing sugar or say substrate concentration. but, DNS method can only give you the reducing sugar concentration.